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JPR Advance Access published online on March 1, 2006
Journal of Plankton Research, doi:10.1093/plankt/fbi147
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received January 9, 2006
Accepted February 24, 2006

Article

Automated detection and enumeration for toxic algae by solid-phase cytometry and the introduction of a new probe for Prymnesium parvum (Haptophyta: Prymnesiophyceae)

Kerstin Töbe 1, Gundula Eller 2, and Linda K. Medlin 1 *

1 Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
2 Present Address: Max Planck Institute for Limnology, August-Thienemann-Strasse 2, 24306 Plön, Germany

* To whom correspondence should be addressed.
Linda K. Medlin, E-mail: lkmedlin{at}awi-bremerhaven.de


  Abstract

Harmful algal blooms have a severe impact on aquaculture and fishery and can be caused by toxic haptophytes and dinoflagellates. Different toxic species, which are not easy to distinguish from their morphologically similar and non-toxic relatives, occur in both groups. Sequencing of the large subunit ribosomal RNA of different strains and taxonomic relatives allowed the design of a probe specific for the toxic Prymnesium parvum. For the rapid detection and enumeration of Prymnesium and Alexandrium cells in cultures and environmental samples, respectively, protocols for fluorescence in situ hybridization (FISH) were adapted for automated detection by a solid phase cytometer, the ChemScan. This cytometer enables the automated counting of fluorescently labelled cells on a membrane filter and subsequently a microscopically verification of these results by the user, because the motorized stage of the microscope is driven to each positive signal by the computer software to localise that cell on the filter. With this fast detection method it was possible to detect, enumerate and verify microalgal cells on a filter, with a detection limit of one cell per membrane filter.

Communicating Editor: KJ Flynn


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