Skip Navigation



JPR Advance Access published online on February 27, 2006
Journal of Plankton Research, doi:10.1093/plankt/fbi145
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
28/6/613    most recent
fbi145v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kim, D.
Right arrow Articles by Oda, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kim, D.
Right arrow Articles by Oda, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received December 19, 2005
Accepted February 23, 2006

Article

Nitric oxide synthase (NOS) like enzyme mediated nitric oxide (NO) generation by harmful red tide phytoplankton, Chattonella marina

Daekyung Kim 1, Kenichi Yamaguchi 1, and Tatsuya Oda 1 *

1 Division of Biochemistry, Faculty of Fisheries, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan

* To whom correspondence should be addressed.
Tatsuya Oda, E-mail: t-oda{at}net.nagasaki-u.ac.jp


  Abstract

The unicellular marine phytoplankton Chattonella marina is known to exhibit potent fish-killing activity. Previous studies have demonstrated that C. marina produces reactive oxygen species (ROS), and ROS-mediated ichthyotoxic mechanism has been postulated. However, the exact toxic mechanism is still controversial. In this study, we obtained evidence that C. marina produces nitric oxide (NO) under normal growth conditions. We utilized chemiluminescence (CL) reaction between NO and luminol-H2O2 to detect NO in C. marina cell suspensions. In this assay, significant CL was observed in C. marina in a cell-number dependent manner, and this was diminished by the addition of carboxy-PTIO, a specific NO scavenger. The NO generation by C. marina was also confirmed by a spectrophotometric assay based on the measurement of the diazo-reaction positive substances (NOx) and by fluorometric assay using highly specific fluorescent indicator of NO. The NO level in C. marina was significantly decreased by L-NAME, a specific NO synthase (NOS) inhibitor. The addition of L-arginine resulted in the increase in NO level, whereas NaNO2 had no effect. These results suggest that a NOS-like enzyme is mainly responsible for NO generation in C. marina.

Communicating Editor: KJ Flynn


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.