Skip Navigation



JPR Advance Access published online on September 6, 2005
Journal of Plankton Research, doi:10.1093/plankt/fbi063
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
27/9/895    most recent
fbi063v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mura, G.
Right arrow Articles by Abatzopoulos, T. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Mura, G.
Right arrow Articles by Abatzopoulos, T. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received April 18, 2005
Accepted August 31, 2005

Article

The use of a multidisciplinary approach for the characterisation of a diploid parthenogenetic Artemia population from Torre Colimena (Apulia, Italy)

Graziella Mura 1, Athanasios D. Baxevanis 2, German Medina Lopez 3, Francisco Hontoria 3, Ilias Kappas 2*, Salvatore Moscatello 4, Giovanni Fancello 1, Francisco Amat 3, and Theodore J. Abatzopoulos 2

1 Dipartimento Di Biologia Animale E Dell’uomo, Universita La Sapienza, Viale Dell’ Universita 32, 00185 Roma, Italia
2 Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
3 Instituto de Acuicultura de Torre de La Sal, 12595 Torre La Sal, Castellon, Spain
4 Dipartimento Di Scienze Tecnologiche Ed Ambientali, Universita Di Lecce, Via Monteroni, 73100 Lecce, Italia

* To whom correspondence should be addressed.
Ilias Kappas, E-mail: kappasi{at}hol.gr


  Abstract

A parthenogenetic Artemia population from Torre Colimena, Southern Italy, originally reported in 1998, is characterised by a multidisciplinary approach including cyst and naupliar biometry, morphometry of adults, scanning electron microscopy of brood pouch, cytogenetics and 16S rRNA PCR-RFLP analysis. We confirmed parthenogenetic status, inferred ploidy level and determined phenotypic and molecular relationships of this population through comparisons with other asexual Artemia strains as well as bisexual species found (A. salina) or introduced (A. franciscana) in the Mediterranean basin. Cyst and naupliar sizes for Torre Colimena are among the smallesxt recorded for asexual Artemia while the opposite is true for chorion thickness. Discriminant analysis of adult body measurements shows increased differentiation (89.5% for the first four out of twelve functions produced) from tetraploid parthenogenetic strains and bisexual species (A. salina, A. franciscana). Scanning electron micrographs of brood pouch reveal the characteristic morphology of asexual strains, while chromosome observations of instar-I nauplii unequivocally establish diploidy. Restriction patterns give evidence that the Torre Colimena population shares an identical set of mitochondrial DNA polymorphisms with a diploid Spanish parthenogenetic strain and it is well differentiated from other tetraploid as well as bisexual auto- and allochthonous strains (A. salina and A. franciscana) from the Mediterranean. The present study may serve as a reference methodological framework for multidisciplinary characterisations and biodiversity assessments in the genus Artemia.

Communicating Editor: KJ Flynn
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.