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JPR Advance Access published online on August 12, 2005
Journal of Plankton Research, doi:10.1093/plankt/fbi053
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© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received June 16, 2005
Accepted August 3, 2005

Article

Effects of fixatives on ciliates as related to cell size

Monica Modigh 1* and Sara Castaldo 2

1 Stazione Zoologica A. Dohrn, Villa Comunale, 80121 Naples Italy
2 Dep. Marine Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131, Ancona, Italy

* To whom correspondence should be addressed.
Monica Modigh, E-mail: modigh{at}szn.it


  Abstract

Abundances of ciliate size classes and of phototrophic ciliates were compared between 2% formaldehyde and 1% Lugol’s preserved samples collected over one year at a coastal Mediterranean site. Large cells and Mesodinuim rubrum were poorly preserved in formaldehyde. A correction factor for ciliate numbers in formaldehyde samples of 1.6 for M. rubrum and of 1.3 for other naked ciliates was found at our study site, where most ciliates were <30µm ESD. Commercially produced Lugols appears inferior to laboratory prepared solutions.

Communicating Editor: KJ Flynn
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