Skip Navigation



JPR Advance Access published online on August 12, 2005
Journal of Plankton Research, doi:10.1093/plankt/fbi052
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
27/8/787    most recent
fbi052v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by N., D.-V.
Right arrow Articles by R., P.-E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by N., D.-V.
Right arrow Articles by R., P.-E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received May 16, 2005
Accepted August 3, 2005

Article

Inhibition of DNA amplification in marine fish larvae preserved in formalin

Díaz-Viloria N. 1, Sánchez-Velasco L. 2, and Perez-Enriquez R. 1*

1 Laboratorio de Genética Acuícola. Centro de Investigaciones Biológicas del Noroeste (CIBNOR). Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur 23090, México
2 Departamento de Plancton y Ecología Marina. Centro Interdisciplinario de Ciencias Marinas (CICIMAR-IPN). La Paz, Baja California Sur 23090, México

* To whom correspondence should be addressed.
Perez-Enriquez R., E-mail: rperez{at}cibnor.mx


  Abstract

Recent advances in molecular biology open the possibility to use formalin-preserved specimens stored in ichthyoplankton collections for population genetics studies. Nine DNA extraction techniques were tested on Engraulis mordax larvae preserved in buffered formalin. However, none of the DNA extracts resulted in the positive amplification of mitochondrial DNA (NADH1, 16srRNA, 12srRNA, and control region fractions). An experiment with different length-time exposure to formalin done with Cynoscion parvipinnis larvae allowed us to confirm the difficulty of amplifying mtDNA from larvae preserved in formalin for long time periods and the possibility of DNA extraction and amplification from short-term (less than 48 h) formalin-fixed marine fish larvae preserved in ethanol (70%). We discuss the possible influence of physical-chemical complexes associated with the duration of preservation to inhibition of amplification reactions.

Communicating Editor: KJ Flynn
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.