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JPR Advance Access published online on November 22, 2004
Journal of Plankton Research, doi:10.1093/plankt/fbh160
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Received August 20, 2004
Accepted November 8, 2004

Article

Development of specific rRNA probes to distinguish between geographic clades of the Alexandrium tamarense species complex

Uwe John 1, Linda K. Medlin 1, and René Groben 2*

1 Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
2 Present address: Centre for Ecology & Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster LA1 4AP, U.K.

* To whom correspondence should be addressed.
René Groben, E-mail: rgro{at}ceh.ac.uk


  Abstract

The globally occurring Alexandrium tamarense/fundyense/catenella species complex consists of toxic and non-toxic strains that are morphologically difficult to distinguish. We developed four specific rRNA probes that can identify the entire species complex, the strains of the toxic North American clade and the strains of the two non-toxic clades from Western Europe and the Mediterranean Sea by DNA dot blot and fluorescence in situ hybridisation (FISH). These probes are a first step for the development of an early warning system for the presence of A. tamarense.


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