Skip Navigation



JPR Advance Access published online on June 10, 2004
Journal of Plankton Research, doi:10.1093/plankt/fbh105
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
26/10/1147    most recent
fbh105v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bodemer, U.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Bodemer, U.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received February 3, 2004
Accepted June 3, 2004

Article

Variability of phycobiliproteins in cyanobacteria detected by delayed fluorescence excitation spectroscopy and its relevance for determination of phytoplankton composition of natural water samples

U. Bodemer 1*

1 Department of Physics, University of Regensburg, Universitätsstr. 31, D-93040 Regensburg, Germany

* To whom correspondence should be addressed. E-mail: ute.bodemer{at}physik.uni-regensburg.de.


  Abstract

Delayed fluorescence (DF) excitation spectroscopy is a fast in vivo method to determine phytoplankton composition in freshwaters using the signature of photosynthetically active pigments which is characteristic in each algal class. Four classes could be distinguished: green algae (including Chlorophyceae, Euglenophyceae and Conjugatophyceae), chromophyte algae (including Bacillariophyceae, Chrysophyceae and Dinophyta), Cryptophyta and cyanobacteria. The DF excitation spectra of various cyanobacteria species were investigated with the aim to improve the reliability of the results obtained by DF. Exposing cyanobacteria to different ambient light conditions did not significantly influence the shape of DF excitation spectra. However, the DF excitation spectra of different cyanobacteria species showed different signatures: a high species-specific variability of the ratio phycocyanin (PC) : phycoerithrin (PE) : allophycocyanin (APC) derived from DF excitation spectra was observed. This variability has to be taken into account determining phytoplankton composition in freshwaters dominated by cyanobacteria using DF. The quality of the fits could be significantly improved using calibration spectra of cyanobacteria species which are according to their spectral features at least similar to those species present in the investigated water body.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J PLANKTON RESHome page
B. A. Hense, P. Gais, U. Jutting, H. Scherb, and K. Rodenacker
Use of fluorescence information for automated phytoplankton investigation by image analysis
J. Plankton Res., May 1, 2008; 30(5): 587 - 606.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.