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JPR Advance Access published online on April 21, 2004
Journal of Plankton Research, doi:10.1093/plankt/fbh075
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Received December 5, 2003
Accepted March 19, 2004

Article

Dynamics and metabolic activity of the benthic cyanobacterium Microcystis aeruginosa in the Grangent reservoir (France)

Delphine Latour 1*, Odile Sabido 2, Marie-José Salençon 3, Hervé Giraudet 1

1 Laboratoire de Biologie Animale et Appliquée, Faculté des Sciences et Techniques Université Jean Monnet, Saint-Etienne, France
2 Centre Commun de Cytométrie en Flux, Faculté de Médecine Université Jean Monnet, Saint-Etienne, France
3 Direction Recherche et Développement - E.D.F., Département LNHE, Chatou, France

* To whom correspondence should be addressed. E-mail: delphine.latour{at}univ-st-etienne.fr.


  Abstract

The dynamics of benthic colonies of Microcystis aeruginosa (cyanobacteria) play an important part in the formation of the summer bloom underlying many harmful effects. Because this benthic phase remains somewhat unknown, we developed an approach using flow cytometry (FCM) to follow the esterase activity of this species in the Grangent reservoir. The esterase activity of benthic cyanobacteria extracted from two layers of sediment (0-2 cm and 2-4 cm deep) was measured, as an indicator of viability, weekly by FCM with carboxyfluorescein diacetate. In parallel, the benthic cyanobacteria concentration was estimated under epifluorescence microscopy; the water temperature and dissolved oxygen concentration were measured in hypolimnion. Esterase activity increased gradually as spring temperatures increased. This metabolic reactivation allowed one part of the benthic cyanobacteria to regain the water column, entailing a simultaneous decrease of their number in the upper sediment (0-2 cm). Then, after a maximum in summer, esterase activity decreased while the number of colonies increased. This corresponded with the domination of the scenescent planktonic form following the decline of the bloom.


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