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JPR Advance Access published online on February 6, 2004
Journal of Plankton Research, doi:10.1093/plankt/fbh028
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Received July 31, 2003;
accepted December 5, 2003

Article

Aminoacyl-tRNA synthetases activity as a growth index in zooplankton

Lidia Yebra 1* and Santiago Hernández-León 2

1

Biological Oceanography Laboratory. Facultad de Ciencias del Mar, Universidad de Las Palmas de Gran Canaria. Campus Universitario de Tafira, 35017, Las Palmas de Gran Canaria. Canary Islands. Spain.

Present Address: Plymouth Marine Laboratory. Prospect Place, The Hoe, Plymouth, PL1 3DH, United Kingdom.


2 Biological Oceanography Laboratory. Facultad de Ciencias del Mar, Universidad de Las Palmas de Gran Canaria. Campus Universitario de Tafira, 35017, Las Palmas de Gran Canaria. Canary Islands. Spain.

* To whom correspondence should be addressed. E-mail: lymo{at}mail.pml.ac.uk.


  Abstract

A simple method for the assay of aminoacyl-tRNA synthetases (AARS) activity was modified for application in planktonic crustaceans as an index of somatic growth. The cladoceran Daphnia magna was cultured at the laboratory and its AARS activity measured without substrate addition. The relationship between the enzyme activity of animals of similar age and individual biomass growing at different rates was tested. A significant relationship was found between aminoacyl-tRNA synthetases activity and somatic growth in terms of both protein and dry weight.


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