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JPR Advance Access published online on November 23, 2009
Journal of Plankton Research, doi:10.1093/plankt/fbp111
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Molecular characterization of bloom-forming Aphanizomenon strains isolated from Vela Lake (Western Central Portugal)

Daniela R. de Figueiredo*, Artur Alves, Mário J. Pereira and António Correia

Department of Biology and CESAM, Centre for Environmental and Marine Studies, University of Aveiro, 3810-193 Aveiro, Portugal

* CORRESPONDING AUTHOR: dfigueiredo{at}ua.pt

Received on May 21, 2009; accepted on October 22, 2009


  Abstract

The diversity within the genus Aphanizomenon (Nostocales, Cyanobacteria) is still controversial and more studies are needed to clarify its heterogeneity. Aphanizomenon strains isolated from the eutrophic shallow Vela Lake (Centralwestern Portugal) during bloom formation in summer months from 2001 to 2007 were characterized combining phenotypic and molecular approaches. The molecular affiliation of the strains was established through sequencing of the 16S rDNA, as well as nifH and hetR genes fragments, and inter-specific genetic variability was checked through repetitive sequences fingerprinting. The morphological characters were characteristic of the descriptions of Aph. issatschenkoi (Cuspidothrix issatschenkoi), Aphanizomenon gracile and Aph. aphanizomenoides. Molecular phylogenetic results corroborated the morphological identification and fingerprinting showed a persistent occurrence of Aph. gracile and Aph. aphanizomenoides strains over the years but also indicated that Aph. aphanizomenoides strains are not clonal within a same bloom. Partial nifH and hetR gene sequences of the cyanobacterial strains showed a high phylogenetic differentiation capacity, even at the strain level.

Corresponding editor: William Li


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