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JOURNAL OF PLANKTON RESEARCH | VOLUME 9 | NUMBER 4 | PAGES 607-612 | 1987
© Oxford University Press

research-article

A system for measuring phytoplankton photosynthesis in a defined light field with an oxygen electrode

Zvy Dubinsky, Paul G. Falkowski1, Anton F. Post2 and Udo M. van Hes2

1Department of Applied Science, Brookhaven National Laboratory Upton, NY 11973, USA 2Laboratorium voor Microbiologie, Universiteit van Amsterdam Niuwe Achtergracht 127, 1018 WS Amsterdam, The Netherlands Department of Life Sciences, Bar-Ilan University Ramat-Gan 52100, Israel

Received on April 4, 1986; accepted on March 13, 1987 A system is described for the precise continuous measurement of oxygen consumption and photo-synthetic oxygen evolution in a well defined light field with a polarographic oxygen electrode. The procedure uses a parallel light beam to irradiate samples, a relatively short pathlength and temperature regulation. Maximal sensitivity of the O2-mcasurement is 1.4 µmol O2/mV at 20°C. The O2-chamber and the O2-amplifier are presented in full detail together with a typical recorder tracing of a photosynthesis-irradiance curve.


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