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JOURNAL OF PLANKTON RESEARCH | VOLUME 9 | NUMBER 1 | PAGES 41-50 | 1987
© Oxford University Press

research-article

Quantification of track and grain density autoradiography and evaluation of 14C loss on preservation

Heath J. Carney1 and Gary L. Fahnenstiel2

2Great Lakes Environmental Research Lab./NOAA 2300 Washtenaw Ave., Ann Arbor, MI 48104, USA Great Lakes Research Division, University of Michigan Ann Arbor, MI 48109

Received on April 1, 1985; accepted on July 1, 1986

Sources of error in quantifying autoradiography were determined with unialgal cultures and natural assemblages. Carbon-14 activity of cells as recorded by track autoradiography was generally not significantly different from scintillation counts. Differences which did occur were apparently due largely to filter-retained extracellular 14C. An analysis of 37 preparations confirmed that tracks per cell for healthy log-phase populations followed a Poisson distribution. Quantification and analysis of intraspecific distributions for the grain density technique were relatively difficult. 14C loss on preservation was lower and more consistent (0–21 %) than previously reported. Autoradiographs indicated that this loss consisted of unfixed filter-retained 14C or an extracellular release product rather than carbon within algal cells. Thus, track autoradiography was more reliable for quantifying species-specific net productivity than other studies have indicated.

1Present address: Division of Environmental Studies and Institute of Ecology, University of California, Davis, CA 95616, USA


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