Skip Navigation


JPR Advance Access originally published online on April 19, 2007
Journal of Plankton Research 2007 29(6):483-493; doi:10.1093/plankt/fbm031
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
29/6/483    most recent
fbm031v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bucklin, A.
Right arrow Articles by Pierson, J. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Bucklin, A.
Right arrow Articles by Pierson, J. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

DNA barcodes for species identification of euphausiids (Euphausiacea, Crustacea)

Ann Bucklin1,*, Peter H. Wiebe2, Sara B. Smolenack3, Nancy J. Copley2, Jason G. Beaudet1, Kaitlin G. Bonner3, Jaime Färber-Lorda4 and James J. Pierson3,{dagger}

1 Department of Marine Sciences, University of Connecticut—avery Point, 1080 Shennecossett Road, Groton, CT 06340, USA 2 Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA 3 Ocean Process Analysis Laboratory, University of New Hampshire, Durham, NH 03824, USA 4 Centro de Investigación Cientifica y de Educación Superior de Ensenada, México

* Corresponding Author: ann.bucklin{at}uconn.edu

Received on September 19, 2006; accepted on April 13, 2007


  Abstract

Many species of euphausiids (Euphausiacea, Crustacea) are distinguished by subtle or geographically variable morphological characters, and erroneous identification of euphausiid species may be more frequent than currently acknowledged. DNA barcodes (short DNA sequences that discriminate species and aid in recognition of unknown species) are of use for this group. A ~650 bp region of mitochondrial cytochrome oxidase I (mtCOI) was sequenced for 40 species of 10 euphausiid genera: Bentheuphausia, Euphausia, Meganyctiphanes, Nematobrachion, Nematoscelis, Nyctiphanes, Stylocheiron, Tessarabrachion, Thyssanoessa and Thysanopoda. mtCOI sequence variation discriminated all species; pairwise differences averaged 16.4% (range 7–24%); mean generalized time reversible (GTR) genetic distance was 26.7%. mtCOI reliably identified euphausiid species: variation within species was typically < 1% and GTR distance was typically < 2%. Atlantic and Pacific Ocean populations of Euphausia brevis differed by 13% (GTR genetic distance = 28%) and may deserve status as distinct species. mtCOI gene trees were reconstructed for five genera using maximum parsimony, maximum likelihood and Bayesian algorithms; best-fit models of nucleotide evolution were determined for each genus. The mtCOI gene tree for 20 species of Euphausia reproduced one of three morphologically defined species groups. mtCOI resolved relationships among closely related species of most genera, usually in accord with morphological groupings. A comprehensive DNA barcode database for euphausiids will help ensure accurate species identification, recognition of cryptic species and evaluation of taxonomically meaningful geographic variation.

{dagger} Present Address: University of Maryland Center for Environmental Science, 2020 Horns Point Road, Cambridge, MD 21613, USA

Communicating editor: K.J. Flynn


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J PLANKTON RESHome page
G. B. McManus and L. A. Katz
Molecular and morphological methods for identifying plankton: what makes a successful marriage?
J. Plankton Res., October 1, 2009; 31(10): 1119 - 1129.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.