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JPR Advance Access originally published online on August 12, 2005
Journal of Plankton Research 2005 27(8):845-849; doi:10.1093/plankt/fbi053
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© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.

SHORT COMMUNICATION

Effects of fixatives on ciliates as related to cell size

Monica Modigh1,* and Sara Castaldo2

1 Stazione Zoologica A. Dohrn, Villa Comunale, 80121 Naples, Italy and 2 Department of Marine Sciences, Polytechnic University of Marche, via Brecce Bianche, 60131 Ancona, Italy

* Corresponding Author: modigh{at}szn.it

Received June 16, 2005; accepted in principle July 26, 2005; accepted for publication August 3, 2005; published online August 12, 2005

Abundances of ciliate size classes and of phototrophic ciliates were compared between 2% formaldehyde and 1% Lugol’s preserved samples collected over 1 year at a coastal Mediterranean site. Large cells and Mesodinuim rubrum were poorly preserved in formaldehyde. A correction factor for ciliate numbers in formaldehyde samples of 1.6 for M. rubrum and of 1.3 for other naked ciliates was found at our study site, where most ciliates were <30 µm equivalent spherical diameter (ESD). Commercially produced Lugol’s appears inferior to laboratory prepared solutions.


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This article has been cited by other articles:


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