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JPR Advance Access originally published online on August 12, 2005
Journal of Plankton Research 2005 27(8):787-792; doi:10.1093/plankt/fbi052
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© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org.

Inhibition of DNA amplification in marine fish larvae preserved in formalin

N. Díaz-Viloria1, L. Sánchez-Velasco2 and R. Perez-Enriquez1,*

1 Laboratorio de Genética Acuícola, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, col. Playa Palo de Santa Rita, la Paz, Baja California sur 23090, México and 2 Departamento de Plancton y Ecología Marina, Centro Interdisciplinario de Ciencias Marinas (CICIMAR-IPN), la Paz, Baja California sur 23090, México

* Corresponding Author: rperez{at}cibnor.mx

Received May 16, 2005; accepted in principle July 08, 2005; accepted for publication August 03, 2005; published online August 12, 2005

Recent advances in molecular biology open the possibility to use formalin-preserved specimens stored in ichthyoplankton collections for population genetics studies. Nine DNA extraction techniques were tested on Engraulis mordax larvae preserved in buffered formalin. However, none of the DNA extracts resulted in the positive amplification of mitochondrial DNA (mtDNA) (NADH1, 16srRNA, 12srRNA and control region fractions). An experiment with different length-time exposure to formalin done with Cynoscion parvipinnis larvae allowed us to confirm the difficulty of amplifying mtDNA from larvae preserved in formalin for long time periods and the possibility of DNA extraction and amplification from short-term (less than 48 h) formalin-fixed marine fish larvae preserved in ethanol (70%). We discuss the possible influence of physical–chemical complexes associated with the duration of preservation to inhibition of amplification reactions.


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