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JPR Advance Access originally published online on October 21, 2004
Journal of Plankton Research 2005 27(1):91-101; doi:10.1093/plankt/fbh154pap
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Journal of Plankton Research Vol. 27 No. 1 © Oxford University Press 2004; all rights reserved

A comparison of HPLC pigment analyses and biovolume estimates of phytoplankton groups in an oligotrophic lake

Teresa Buchaca1,2,*, Marisol Felip1 and Jordi Catalan2

1 Departament D’Ecologia I Centre de Recerca D’Alta Muntanya, Universitat de Barcelona, Avgda. Diagonal 645, E-08028 Barcelona, Spain and 2 Centre D’Estudis AvanÇats de Blanes (CEAB-CSIC), Accés Cala St. Francesc 14, E-17300 Blanes, Spain

* Corresponding Author: buch{at}ceab.csic.es

Received May 26, 2004; accepted in principle August 27, 2004; accepted for publication September 23, 2004; published online October 21, 2004

Assessment of the contribution of distinct algal groups to phytoplankton biomass in oligotrophic lakes by marker pigments is compared with assessment by cell-counting biovolume estimates. Seasonal samples from an oligotrophic alpine lake (Redon, Pyrenees) mostly included species of chrysophytes, dinoflagellates, cryptophytes and chlorophytes. The chlorophyl a (Chl a) corresponding to each algal group was estimated using HPLC pigment analyses and the CHEMTAX program. Chl a estimates and biovolume showed a significant correlation for all the groups during the ice-free season except for chlorophytes. However, some of the samples from the initial phase of the ice cover presented a clear departure from the relationship during the ice-free period in most groups. On the other hand, the ratios between a specific marker pigment and the biovolume of the marked algal group were significantly constant within the photic zone (>1% surface irradiance) for most of the pigments and groups, including chlorophytes. Nevertheless, the ratios increased and showed a large variability for samples below the photic depth or below the ice cover. The violaxanthin-chrysophyte biovolume ratio presented an opposed tendency to other pigment-biovolume ratios, which increased in inverse proportion to the depth of the sample. The results are discussed in terms of methodological limitations, acclimation responses and species composition.


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