Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Neumüller, M.
Right arrow Articles by Mckee, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Neumüller, M.
Right arrow Articles by Mckee, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Plankton Research Vol.24 no.8 pp.741-746, 2002
© Oxford University Press 2002

Assessment of a microscopic photobleaching technique for measuring the spectral absorption efficiency of individual phytoplankton cells

Mathias Neumüller, Alex Cunningham,* and David Mckee

Department Of Physics And Applied Physics, University Of Strathclyde, 107 Rottenrow, Glasgow G4 0ng, UK

* a.cunningham{at}strath.ac.uk

Measurements of the absorptance of photosynthetic pigments in individual phytoplankton cells were made using an epifluorescence microscope equipped with a spectrograph and CCD array detector. Correction for light loss due to scattering was achieved by bleaching the cells with intense light from a mercury arc lamp, and using the bleached cells as a spectrophotometric blank. Absorption efficiency factors were calculated from knowledge of the geometrical cross-section of the cells obtained fromcalibrated video images acquired at the time of measurement. The single-cell efficiency factors were consistent with the average absorption efficiencies of cell suspensions measured using a spectrophotometer over most of the visible spectrum, but they were significantly lower below 420 nm. Cells of the diatom Cyclotella cryptica and the chlorophyte Chlorella salina showed clear spectral differences in spectral shape that could be related to taxonomic differences in pigment content, but absorption efficiency factors of approximately 0.4 at 675 nm were found for both species.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.