Journal of Plankton Research, Vol 21, 1877-1889, Copyright © 1999 by Oxford University Press
C Carpentier, H Ketelaars, A Wagenvoort and K Pikaar-Schoonen
A semi-automated biovolume measurement application called BACCHUS
(Biovolume Analysis Cum Calculus and High Utility Statistics), based on an
image-analysis program combined with a database, was developed. With this
application, biovolumes of plankton taxa can be determined while counting
routine samples. Statistical analyses of the ln-transformed results are
performed on-line. The width of the 95% confidence interval is calculated
and indicates the precision of the calculated mean. By setting limits to
the maximum allowed width of the interval, the extent of desired precision
can be defined. For our laboratory, we decided that the limits of the
confidence interval must be <0.2 ln units above or below the mean of
the ln-transformed results. As soon as this criterion is reached, the user
can stop measuring, thereby minimizing the number of measurements necessary
to reach a mean biovolume which is sufficiently precise. Other advantages
of the BACCHUS application are a very flexible input of master data, output
of stored data and a user-friendly operation environment. BACCHUS is very
easy to customize because it does not require any expertise in database
programming.
ORIGINAL ARTICLES
Rapid and versatile routine measurements of plankton biovolumes with BACCHUS
Biological Laboratory, Water Storage Company Brabantse Biebosch, PO Box 61, NL-4250 DB Werkendam, The Netherlands; Present address: Kiwa Research and Consultancy, PO Box 1072, NL-3430 BB Nieuwegein, The Netherlands; Corresponding author
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