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JOURNAL OF PLANKTON RESEARCH | VOLUME 20 | NUMBER 9 | PAGES 1699-1709 | 1998
© Oxford University Press

research-article

Measurement of light absorption by aquatic particles retained on filters: determination of the optical pathlength amplification by the ‘transmittance-reflectance’ method

Stelvio Tassan and Giovanni M Ferrari

Space Applications Institute, CEQ Joint Research Centre-Ispra Site Ispra (VA), Italy

Received on March 1, 1998; accepted on April 25, 1998 The optical pathlength amplification (ß factor), induced by multiple scattering in glass-fibre filters used for light absorption measurements of aquatic particles, has been measured by the transmittance-reflectance (T-R) method for algal species with cell diameter in the range from 1 to 5 µm. Theß factors obtained showed a much lower dispersion than that observed in similar measurements carried out by the standard transmittance method, suggesting that this could be due, at least in part, to the incomplete account of the scattering losses taken by the latter method. An experimental test has verified that the ß factor expression derived from measurements carried out by the T-R method on an algal culture is also applicable to detrital particles.


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