JOURNAL OF PLANKTON RESEARCH | VOLUME 18 | NUMBER 7 | PAGES 1261-1267 | 1996
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Use of the 0.22 µm Millipore membrane for light-transmission measurements of aquatic particles
Institute for Remote Sensing Applications, Commission of the European Communities, Joint Research Centre Ispra Site, 1-21020 Ispra (VA), Italy
Received on October 12, 1995; accepted on February 7, 1996 The use of the 0.22 µm Millipore cellulose membrane for the measurement of the in vivo absorption of aquatic particles by the light-transmission method has been explored relative to the performance of the currently used 0.7 µm Whatman GF/F glass-fiber filter. The standard solvent extraction process for pigment removal has been replaced by a newly developed process based on NaClO bleaching. The tests carried out indicate that the two filters exhibit comparable performances. It has been shown that a double measurement, performed first in the particles retained in the GF/F filter and then on the sample obtained passing the GF/F filtrate through a Millipore membrane, allows for the selective determination of the absorption of the particle fraction with diameter in the 0.220.7 µm range.
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