Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hernández-Saavedra, N. Y.
Right arrow Articles by Vazquez-Dulhalt, R.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hernández-Saavedra, N. Y.
Right arrow Articles by Vazquez-Dulhalt, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

JOURNAL OF PLANKTON RESEARCH | VOLUME 17 | NUMBER 1 | PAGES 59-69 | 1995
© Oxford University Press

research-article

Osmotic adjustment in marine yeast

Norma Y. Hernández-Saavedra, Jose Luis Ochoa and Rafael Vazquez-Dulhalt1

Center for Biological Research PO Box 128, La Paz, Baja California Sur, 23000 Mexico 1Department of Microbiology, University of Alberta Edmonton, Alberta, T6G 2E9, Canada

Received on January 22, 1993; accepted on August 30, 1994 The effect of environmental salinity on cell growth, and on the composition and accumulation of compatible solutes, or osmotica, of five yeast strains (Aureobasidium pullulans, Candida sp., Cryptococcus albidus var. albidus, Debaryomyces hansenii and Rhodotorula rubra) was compared. All these yeast were isolated from manne environments, but were able to grow in the absence of salt and should therefore be considered as halotolerant strains. According to their specific cell growth rates at different salt concentrations, these strains vary in their capacity to osmotically adjust to modifications in external salinity. Candida sp. appears to be the most sensitive since the maximum salt concentration at which it can grow is 1.54 mol 1-1 NaCl; however, it showed the highest specific cell growth in the range of 0 to 1.54 mol 1-1 NaCl. Aureobasidium pullulans, on the other hand, showed the lowest specific growth rate, but the highest halotolerance range from 0 to 5.13 mol 1-1 NaCl. Debaryomyces hansenii, in contrast, showed higher specific growth at this salinity range Cryptococcus albidus var. albidus and Rhodotorula rubra showed similar specific cell growth rate values and halotolerance between 0 and 2.45 mol 1-1 NaCl. The protein and carbohydrate content of the biomass of the different yeast cells, as a result of external salinity vanation, remained practically constant. The most important effects of the increase in salt concentration in the culture medium were the reduction of cell volume and the accumulation of low-molecular-weight metabolites (LMWM). which appear to act as osmoregulators. Glycerol was found as the major compatible solute in the different marine yeasts studied herein with a total contribution of 64–96% of the internal cell osmolarity. Other LMWM, like carbohydrates and amino acids, contributed to a lesser extent to compensate for the rise in osmotic pressure promoted by the salinity of the external environment.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
T. Kogej, J. Ramos, A. Plemenitas, and N. Gunde-Cimerman
The Halophilic Fungus Hortaea werneckii and the Halotolerant Fungus Aureobasidium pullulans Maintain Low Intracellular Cation Concentrations in Hypersaline Environments
Appl. Envir. Microbiol., November 1, 2005; 71(11): 6600 - 6605.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.